Simultaneous measurement of several parameters in one sample
The trend towards personalized medicine requires better and more and more frequent diagnostic testing. Multiplexed analysis is a technique for making diagnostic testing faster and more affordable. The key to multiplexing is miniaturization: simultaneous detection and analysis of different analytes in the same reaction vessel using the same sample. Combining several tests results in significant savings in time and money.
Our Microarray Analyzers are designed for low density planar arrays with up to a couple of hundred multiplexed measurements. Customized microarray printing by one of our partners offers open configuration of panels and definition of consumables. The microarray might be located at the bottom of the wells of a 96-well plate, on glass slides, special biochips, or other custom formats. The 96-well format is ideally-suited for multiplexing ELISA assays because existing liquid handling equipment can be used.
Typical multiplexed assays include genotyping, DNA arrays, protein arrays, and multiplexed immunoassays, such as ELISAs. We are working flexibly with a network of experienced partners to create the best multiplexing solution for each customer.
Several applications are predestined for multiplexed diagnostics, depending on the amount of markers needed for diagnosis. Typical panels are created for diagnostic low-density microarray products:
- Infectious Diseases
- Assays for simultaneous determination of the presence or absence of certain pathogenic microorganisms in one sample
- Mostly DNA based assays after PCR, often qualitative
- E.g. testing of HPV, Respiratory Viruses, Borreliosis, Chagas disease
- Autoimmune Diseases
- Assays to determine multiple biomarkers in one sample
- Protein based, mostly quantitative
- E.g. testing for Rheumatoid Arthritis, Celiac Disease, Allergies
- HLA typing
- Blood Screening
- Early stage testing of different cancer types
Application notes from SensoSpot® customers
With 15 years of experience in microarray analysis, We work together with companies from the Life Science and Diagnostics sector throughout the entire product life cycle. The result is a cost-efficient and application-tailored IVD product for multiplexed diagnostics.
The Detectx-Rv test is a multiplexed viral diagnostic assay for the detection of SARS-CoV-2. It contains up to 12 specific probes and will differentiate Corona-like-viruses from SARS-CoV-2, delivering industry leading accuracy. The DNA-based fluorescent microarray is read and analyzed using the SensoSpot® Fluorescene.
The RIDA® Chip FoodGuide test is a microarray enzyme immunoassay for in-vitro diagnostic use. It is suitable for the quantitative detection of specific IgG antibodies against up to 100 food allergens in human blood samples. This enables diagnosis of delayed food allergies. The SensoSpot® Colorimetry is part of the diagnostic product.
Simian SARS-CoV-2 test is an in vitro serological test using a multiplex microarray of SARS-CoV-2 specific antigens to probe serum for presence of IgG antibodies intended for use by veterinarians and animal care staff to identify animals previously exposed to the virus. Detection of SARS-CoV-2 specific antibodies indicates current of prior infection and is carried out with the SensoSpot® Colorimetry.
Specific Borrelia IgG and lgM antibodies can be detected in human blood using the ViraChip® Test Kit from Viramed. It allows for diagnosis of human Borrelia infection. The colorimetric enzyme-immunoassay is read and analyzed using the SensoSpot® Colorimetry Reflex.